Puracyp Inc
Puracyp is dedicated to providing high quality research with a primary focus on high volume screening of new chemical entities to identify potential inducers of P450 enzymes and ABC transporters. Puracyp’s nuclear receptor activation service utilizes either stably integrated cell lines or transiently transfected cells harboring P450 enhancer elements in reporter gene constructs and human or animal nuclear receptors including PXR, CAR and AhR. Nuclear receptor assays currently available include those employing the CYP3A, CYP2B, P-glycoprotein or CYP1A2-DRE enhancers and promoters.
Nuclear receptors derived from various species are also available that can alleviate many animal studies and provide valuable information regarding a NCE and its potential to alter pharmacokinetic parameters. Information gained from employing human nuclear receptors can be used to predict in vivo responses from NCEs with regards to drug-drug interactions and adverse drug effects.
Puracyp’s scientists are extensively published and have been involved in human P450 metabolism research for a number of years. Presently, the company has two main divisions, products and services. Products now include kits that are all inclusive and contain our stably integrated human or rat PXR and human AhR cell lines. Puracyp also offers vials of our cryopreserved DPX2, 1A2-DRE and rPXR cells in 6 or 12 million cells per vial that can be formatted for 96 or 384 well plate analyses.